| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
09/11/2010 |
|
Data da última atualização: |
20/05/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
DAL BOSCO, D.; SINSKI, I.; RITSCHEL, P. S.; QUECINI, V. |
|
Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; VERA MARIA QUECINI, CNPUV. |
|
Título: |
Residues of 2,4-Dichlorophenoxyacetic acid in plant-tissue culture glassware: their effects and a decontamination protocol. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
International Journal of Biotechnology & Biochemistry, Delhi, v. 6, n. 4, p. 617-624, 2010. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Plant development and gene transfer studies frequently require the induction of quiescent somatic cells to recuperate embryogenic potential via application of synthetic auxin analogs. The severe effects of hormone analogs on plant growth and development have prompted their use as herbicides in agriculture, where they were demonstrated to be deleterious contaminants to soil and water, also associated to increased frequencies of certain human tumors. In order to investigate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) contamination in plant tissue-culture laboratories, the development of three plant species was investigated on glassware free of hormone residues and plates containing synthetic auxin residue. Residual 2,4-D severely impaired seed germination, seedling establishment and aerial and root development of the tested plant species, namely; Arabidopsis thaliana, Petunia x hybrida and Solanum lycopersicum. A simple and feasible decontamination protocol for laboratory plastic and glassware was developed, based on international recommendations for handling chlorophenoxy herbicide residues. The effectiveness of the decontamination procedure was assayed by in vivo analyses of plant growth and development on hormone-free media contained in contaminated, decontaminated and new glassware. Plant development parameters evaluated on decontaminated glassware were indistinguishable from those observed in plants grown on new plates, indicating that hormone contamination was significantly reduced to levels unable to affect the plants for the three species investigated. MenosPlant development and gene transfer studies frequently require the induction of quiescent somatic cells to recuperate embryogenic potential via application of synthetic auxin analogs. The severe effects of hormone analogs on plant growth and development have prompted their use as herbicides in agriculture, where they were demonstrated to be deleterious contaminants to soil and water, also associated to increased frequencies of certain human tumors. In order to investigate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) contamination in plant tissue-culture laboratories, the development of three plant species was investigated on glassware free of hormone residues and plates containing synthetic auxin residue. Residual 2,4-D severely impaired seed germination, seedling establishment and aerial and root development of the tested plant species, namely; Arabidopsis thaliana, Petunia x hybrida and Solanum lycopersicum. A simple and feasible decontamination protocol for laboratory plastic and glassware was developed, based on international recommendations for handling chlorophenoxy herbicide residues. The effectiveness of the decontamination procedure was assayed by in vivo analyses of plant growth and development on hormone-free media contained in contaminated, decontaminated and new glassware. Plant development parameters evaluated on decontaminated glassware were indistinguishable from those observed in plants grown on new plates, indicating that hormone contamination was ... Mostrar Tudo |
|
Palavras-Chave: |
Cultura de tecidos; Embriogênese somática. |
|
Thesagro: |
Auxina; Biologia; Contaminação; Genética; Herbicida; Propagação vegetativa. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195342/1/RESIDUOS.pdf
|
|
Marc: |
LEADER 02388naa a2200253 a 4500
001 1866500
005 2019-05-20
008 2010 bl uuuu u00u1 u #d
100 1 $aDAL BOSCO, D.
245 $aResidues of 2,4-Dichlorophenoxyacetic acid in plant-tissue culture glassware$btheir effects and a decontamination protocol.$h[electronic resource]
260 $c2010
520 $aPlant development and gene transfer studies frequently require the induction of quiescent somatic cells to recuperate embryogenic potential via application of synthetic auxin analogs. The severe effects of hormone analogs on plant growth and development have prompted their use as herbicides in agriculture, where they were demonstrated to be deleterious contaminants to soil and water, also associated to increased frequencies of certain human tumors. In order to investigate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) contamination in plant tissue-culture laboratories, the development of three plant species was investigated on glassware free of hormone residues and plates containing synthetic auxin residue. Residual 2,4-D severely impaired seed germination, seedling establishment and aerial and root development of the tested plant species, namely; Arabidopsis thaliana, Petunia x hybrida and Solanum lycopersicum. A simple and feasible decontamination protocol for laboratory plastic and glassware was developed, based on international recommendations for handling chlorophenoxy herbicide residues. The effectiveness of the decontamination procedure was assayed by in vivo analyses of plant growth and development on hormone-free media contained in contaminated, decontaminated and new glassware. Plant development parameters evaluated on decontaminated glassware were indistinguishable from those observed in plants grown on new plates, indicating that hormone contamination was significantly reduced to levels unable to affect the plants for the three species investigated.
650 $aAuxina
650 $aBiologia
650 $aContaminação
650 $aGenética
650 $aHerbicida
650 $aPropagação vegetativa
653 $aCultura de tecidos
653 $aEmbriogênese somática
700 1 $aSINSKI, I.
700 1 $aRITSCHEL, P. S.
700 1 $aQUECINI, V.
773 $tInternational Journal of Biotechnology & Biochemistry, Delhi$gv. 6, n. 4, p. 617-624, 2010.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 4 | |
| Registros recuperados : 4 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
